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QVQ
Functionalization

Functionalization.

QVQ routinely equips single-domain antibodies with a proprietary C-Direct tag at the C-terminus.

Single-domain antibody functionalization.

QVQ routinely equips single-domain antibodies with a proprietary C-Direct tag at the C-terminus.

This low immunogenic tag allows for good production in yeast, affinity purification, and contains an unpaired thiol for directional conjugation to a wide range of labels.

Possible functionalizations of these sdAbs include genetic fusions and site-directed conjugations via maleimide-thiol reactions to the following molecules.

Fluorophores

  • HiLyte488
  • HiLyte555
  • HiLyte647
  • IRDye 680RD
  • IRDye 800CW
  • Various ATTO dyes

Chelators

  • DOTA
  • NOTA
  • DOTAGA

Proteins

  • HRP
  • Luciferases
  • Fluorophores

Other

  • Biotin
  • Peptides
  • Oligonucleotides

Alternatively, we offer sdAb-DBCO conjugates for conjugation via Click Chemistry to azide-modified labels including fluorophores of different wavelengths. In addition, we offer custom conjugations to your sdAb and label of interest.

Service sheets.

Fluorophores

Chelators

HRP

Biotin

Peptides

Oligos

DBCO

Variety of applications.

These modifications allow for a variety of applications. In this application note, we elaborate on examples of applications of fluorescent single-domain antibodies.

sdAb conjugates application note
Other functionalizations can be introduced by genetic fusion of recombinant proteins. QVQ offers the generation of multispecific and multivalent single-domain antibody constructs. In addition, we can fuse single-domain antibodies to different Fc-domains, peptides, or proteins. Other fusion proteins can be generated upon request. Please contact us for more information.