Single-domain antibody functionalization
QVQ routinely equips single-domain antibodies with a proprietary C-Direct tag at the C-terminus.
This low immunogenic tag allows for good production in yeast, affinity purification, and contains an unpaired thiol for directional conjugation to a wide range of labels.
QVQ offers conjugation services in which tagged single domain antibody are directionally conjugated to:
– fluorescent dyes (e.g. Hilyte Fluor, IRDye)
– chelators (e.g. DOTA, NOTA)
– biotin
– peptides
– oligonucleotides
– proteins (e.g. HRP, luciferases, or fluorophores)
Other functionalizations can be introduced by genetic fusion. QVQ offers the generation of multispecific and multivalent single-domain antibody constructs. In addition, we can fuse single-domain antibodies to different Fc-domains, peptides, or proteins. Other fusion proteins can be generated upon request. Please contact us for more information.
These modifications allow for a variety of applications. Below are examples of functionalized sdAbs and their applications.
In this application note, we elaborate on the applications of fluorescent single-domain antibodies.
