NanoB2 for assessing binding of ligands to drug targets

In a collaborative project, researchers at QVQ (Stephanie Mareike Anbuhl and Raimond Heukers), VU Amsterdam (Martine Smit lab) and University of Nottingham (Laura Kilpatrick and Steve Hill) have developed a novel approach using labeled VHH to easily assess and quantify binding of ligands to different drug targets. This technology, named NanoB2 (nanobody-NanoBRET), was published in the journal of Cell Reports Methods.

The work, driven by Jelle van den Bor and Nick Bergkamp proves again the high potential of labeled VHH as detection agents. In this new technology, labeled VHH are used for the determination of the binding sites, kinetics and affinities of unlabeled drugs and thereby aid the identification of novel ligands.