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Disruption of basal CXCR4 oligomers impairs oncogenic properties in lymphoid neoplasms
Mobach S, Bergkamp ND, Ma Z, Haselager MV, Anbuhl SM, Jurriens D, van den Bor J, Wang Z, Crudden C, Roos JL, Perez Almeria CV, Boerjonge RA, Lohse MJ, Bosma R, Eldering E, Siderius M, Wu W, Spaargaren M, Tonino SH, Kater AP, Smit MJ, Heukers R.
bioRxiv 2024. doi: 10.1101/2024.12.06.627249

Constitutive activity of an atypical chemokine receptor revealed by inverse agonistic nanobodies
Perez Almera CV, Otun O, Schlimgen R, Lamme TD, Crudden C, Youssef N, Musli L, Jenjak S, Bobkov V, Drube J, Hoffmann C, Volkman BF, Granier S, Bechara C, Siderius M, Heukers R, Schafer CT, Smit MJ.
bioRxiv 2024. doi: 10.1101/2024.11.04.621790

Inhibition of HIV-1 replication by nanobodies targeting tetraspanin CD9
Umotoy JC, Kroon PZ, Man S, van Dort KA, Atabey T, Schriek AI, Dekkers G, Herrera-Carrillo E, Geijtenbeek TBH, Heukers R, Kootstra NA, van Gils MJ, de Taeye SW. 
iScience. 2024 Oct 18. doi: doi.org/10.1016/j.isci.2024.110958.

Multivalent CXCR4-targeting nanobody formats differently affect affinity, receptor clustering, and antagonism
Anbuhl SM, Dervillez X, Neubacher S, Schriek AI, Bobkov V, de Taeye SW, Szpakowska M, Siderius M, Grossmann TN, Chevigné A, Smit MJ, Heukers R.
Biochem Pharmacol. 2024 Sept. doi: 10.1016/j.bcp.2024.116457.

Nanobody-liposomes as novel cancer vaccine platform to efficiently stimulate T cell immunity
Bouma RG, Nijen Twilhaar MK, Brink HJ, Affandi AJ, Mesquita BS, Olesek K, van Dommelen JMA, Heukers R, de Haas AM, Kalay H, Ambrosini M, Metselaar JM, van Rooijen A, Storm G, Oliveira S, van Kooyk Y, den Haan JM.
Int J Pharm. 2024 Jul. doi: 10.1016/j.ijpharm.2024.124254.

Inhibition of cleavage of human complement component C5 and the R885H C5 variant by two distinct high affinity anti-C5 nanobodies
Struijf EM, De la O Becerra KI, Ruyken M, de Haas CJC, van Oosterom F, Siere DY, van Keulen JE, Heesterbeek DAC, Dolk E, Heukers R, Bardoel BW, Gros P, Rooijakkers SHM.
JBC. 2023 Aug. doi: 10.1016/j.jbc.2023.104956.

A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
Jeremiasse B, van Ineveld RL, Bok V, Kleinnijenhuis M, de Blank S, Alieva M, Johnson HR, van Vliet EJ, Zeeman AL, Wellens LM, Llibre-Palomar G, Barrera Román M, Di Maggio A, Dekkers JF, Oliveira S, Vahrmeijer AL, Molenaar JJ, Wijnen MHWA, van der Steeg AFW, Wehrens EJ, Rios A, Anne C.
EMCO. 2024 Jun 3. doi:10.1038/s44321-024-00084-4.

NanoB² to monitor interactions of ligands with membrane proteins by combining nanobodies and NanoBRET
van den Bor J, Bergkamp ND, Anbuhl SM, Dekker F, Comez D, Perez Almeria CV, Bosma R, White CW, Kilpatrick LE, Hill SJ, Siderius M, Smit MJ, Heukers R.
Cell Rep Methods. 2023 Mar 27. doi: 10.1016/j.crmeth.2023.100422.

Candidalysin Is the Hemolytic Factor of Candida albicans.
Mogavero S, Höfs S, Lauer AN, Müller R, Brunke S, Allert S, Gerwien F, Groth S, Dolk E, Wilson D, Gutsmann T, Hube B.
Toxins (Basel). 2022 Dec 15;14(12):874. doi: 10.3390/toxins14120874.

Fluorescently tagged nanobodies and NanoBRET to study ligand-binding and agonist-induced conformational changes of full-length EGFR expressed in living cells.
Comez D, Glenn J, Anbuhl SM, Heukers R, Smit MJ, Hill SJ, Kilpatrick LE.
Front Immunol. 2022 Nov 23;13:1006718. doi: 10.3389/fimmu.2022.1006718. eCollection 2022.

Novel VHH-Based Tracers with Variable Plasma Half-Lives for Imaging of CAIX-Expressing Hypoxic Tumor Cells.
van Lith SAM, Huizing FJ, Franssen GM, Hoeben BAW, Lok J, Doulkeridou S, Boerman OC, Gotthardt M, van Bergen En Henegouwen PMP, Bussink J, Heskamp S.
Mol Pharm. 2022 Oct 3;19(10):3511-3520. doi: 10.1021/acs.molpharmaceut.1c00841. Epub 2022 Jan 19.

Directing HIV-1 for degradation by non-target cells, using bi-specific single-chain llama antibodies.
Stam JC, de Maat S, de Jong D, Arens M, van Lint F, Gharu L, van Roosmalen MH, Roovers RC, Strokappe NM, Wagner R, Kliche A, de Haard HJ, van Bergen En Henegouwen PM, Nijhuis M, Verrips CT.
Sci Rep. 2022 Aug 4;12(1):13413. doi: 10.1038/s41598-022-15993-y.

Conformational selection guides β-arrestin recruitment at a biased G protein-coupled receptor.
Kleist AB, Jenjak S, Sente A, Laskowski LJ, Szpakowska M, Calkins MM, Anderson EI, McNally LM, Heukers R, Bobkov V, Peterson FC, Thomas MA, Chevigné A, Smit MJ, McCorvy JD, Babu MM, Volkman BF.
Science. 2022 Jul 8;377(6602):222-228. doi: 10.1126/science.abj4922. Epub 2022 Jul 7.

Anti-HIV-1 Nanobody-IgG1 Constructs With Improved Neutralization Potency and the Ability to Mediate Fc Effector Functions.
Schriek AI, van Haaren MM, Poniman M, Dekkers G, Bentlage AEH, Grobben M, Vidarsson G, Sanders RW, Verrips T, Geijtenbeek TBH, Heukers R, Kootstra NA, de Taeye SW, van Gils MJ.
Front Immunol. 2022 May 16;13:893648. doi: 10.3389/fimmu.2022.893648. eCollection 2022.

Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis.
Gangaiah D, Ryan V, Van Hoesel D, Mane SP, Mckinley ET, Lakshmanan N, Reddy ND, Dolk E, Kumar A.
Microbiologyopen. 2022 Apr;11(2):e1270. doi: 10.1002/mbo3.1270.

Structural insights into the non-inhibitory mechanism of the anti-EGFR EgB4 nanobody.
Zeronian MR, Doulkeridou S, van Bergen En Henegouwen PMP, Janssen BJC.
BMC Mol Cell Biol. 2022 Mar 1;23(1):12. doi: 10.1186/s12860-022-00412-x.

Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans.
Mogavero S, Sauer FM, Brunke S, Allert S, Schulz D, Wisgott S, Jablonowski N, Elshafee O, Krüger T, Kniemeyer O, Brakhage AA, Naglik JR, Dolk E, Hube B.
Cell Microbiol. 2021 Oct;23(10):e13378. doi: 10.1111/cmi.13378. Epub 2021 Jul 20.

Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D.
van Ineveld RL, Kleinnijenhuis M, Alieva M, de Blank S, Barrera Roman M, van Vliet EJ, Martínez Mir C, Johnson HR, Bos FL, Heukers R, Chuva de Sousa Lopes SM, Drost J, Dekkers JF, Wehrens EJ, Rios AC.
Nat Biotechnol. 2021 Oct;39(10):1239-1245. doi: 10.1038/s41587-021-00926-3. Epub 2021 Jun 3.

Homogeneous tumor targeting with a single dose of HER2-targeted albumin-binding domain-fused nanobody-drug conjugates results in long-lasting tumor remission in mice.
Xenaki KT, Dorrestijn B, Muns JA, Adamzek K, Doulkeridou S, Houthoff H, Oliveira S, van Bergen En Henegouwen PM.
Theranostics. 2021 Mar 13;11(11):5525-5538. doi: 10.7150/thno.57510. eCollection 2021.

Anti-CfaE nanobodies provide broad cross-protection against major pathogenic enterotoxigenic Escherichia coli strains, with implications for vaccine design.
Amcheslavsky A, Wallace AL, Ejemel M, Li Q, McMahon CT, Stoppato M, Giuntini S, Schiller ZA, Pondish JR, Toomey JR, Schneider RM, Meisinger J, Heukers R, Kruse AC, Barry EM, Pierce BG, Klempner MS, Cavacini LA, Wang Y.
Sci Rep. 2021 Feb 2;11(1):2751. doi: 10.1038/s41598-021-81895-0.

Advanced fluorescence microscopy reveals disruption of dynamic CXCR4 dimerization by subpocket-specific inverse agonists.
Işbilir A, Möller J, Arimont M, Bobkov V, Perpiñá-Viciano C, Hoffmann C, Inoue A, Heukers R, de Graaf C, Smit MJ, Annibale P, Lohse MJ.
Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29144-29154. doi: 10.1073/pnas.2013319117. Epub 2020 Nov 4.

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies.
Soave M, Heukers R, Kellam B, Woolard J, Smit MJ, Briddon SJ, Hill SJ.
Cell Chem Biol. 2020 Oct 15;27(10):1250-1261.e5. doi: 10.1016/j.chembiol.2020.06.006. Epub 2020 Jun 30.

Dual Targeting of Endothelial and Cancer Cells Potentiates In Vitro Nanobody-Targeted Photodynamic Therapy.
Mashayekhi V, Xenaki KT, van Bergen En Henegouwen PMP, Oliveira S.
Cancers (Basel). 2020 Sep 23;12(10):2732. doi: 10.3390/cancers12102732.

Stimulation of Collateral Vessel Growth by Inhibition of Galectin 2 in Mice Using a Single-Domain Llama-Derived Antibody.
Hollander MR, Jansen MF, Hopman LHGA, Dolk E, van de Ven PM, Knaapen P, Horrevoets AJ, Lutgens E, van Royen N.
J Am Heart Assoc. 2019 Oct 15;8(20):e012806. doi: 10.1161/JAHA.119.012806. Epub 2019 Oct 9.

Nanobodies that Neutralize HIV.
Weiss RA, Verrips CT.
Vaccines (Basel). 2019 Jul 31;7(3):77. doi: 10.3390/vaccines7030077.

Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells.
De Groof TWM, Mashayekhi V, Fan TS, Bergkamp ND, Sastre Toraño J, van Senten JR, Heukers R, Smit MJ, Oliveira S.
Mol Pharm. 2019 Jul 1;16(7):3145-3156. doi: 10.1021/acs.molpharmaceut.9b00360. Epub 2019 Jun 19.

Super Potent Bispecific Llama VHH Antibodies Neutralize HIV via a Combination of gp41 and gp120 Epitopes.
Strokappe NM, Hock M, Rutten L, Mccoy LE, Back JW, Caillat C, Haffke M, Weiss RA, Weissenhorn W, Verrips T.
Antibodies (Basel). 2019 Jun 18;8(2):38. doi: 10.3390/antib8020038.

VHH-Photosensitizer Conjugates for Targeted Photodynamic Therapy of Met-Overexpressing Tumor Cells.
Heukers R, Mashayekhi V, Ramirez-Escudero M, de Haard H, Verrips TC, van Bergen En Henegouwen PMP, Oliveira S.
Antibodies (Basel). 2019 Apr 4;8(2):26. doi: 10.3390/antib8020026.

Nanobodies detecting and modulating GPCRs outside in and inside out.
Heukers R, De Groof TWM, Smit MJ.
Curr Opin Cell Biol. 2019 Apr;57:115-122. doi: 10.1016/j.ceb.2019.01.003. Epub 2019 Mar 5.

Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs.
De Groof TWM, Bobkov V, Heukers R, Smit MJ.
Mol Cell Endocrinol. 2019 Mar 15;484:15-24. doi: 10.1016/j.mce.2019.01.021. Epub 2019 Jan 26.

CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry.
Van Hout A, Klarenbeek A, Bobkov V, Doijen J, Arimont M, Zhao C, Heukers R, Rimkunas R, de Graaf C, Verrips T, van der Woning B, de Haard H, Rucker JB, Vermeire K, Handel T, Van Loy T, Smit MJ, Schols D.
Biochem Pharmacol. 2018 Dec;158:402-412. doi: 10.1016/j.bcp.2018.10.015. Epub 2018 Oct 17.

Extremely thin layer plastification for focused-ion beam scanning electron microscopy: an improved method to study cell surfaces and organelles of cultured cells.
VAN Donselaar EG, Dorresteijn B, Popov-Čeleketić D, VAN DE Wetering WJ, Verrips TC, Boekhout T, Schneijdenberg CTWM, Xenaki AT, VAN DER Krift TP, Müller WH.
J Microsc. 2018 Jun;270(3):359-373. doi: 10.1111/jmi.12694. Epub 2018 Mar 25.

Towards an ultra-rapid smartphone- connected test for infectious diseases.
Turbé V, Gray ER, Lawson VE, Nastouli E, Brookes JC, Weiss RA, Pillay D, Emery VC, Verrips CT, Yatsuda H, Athey D, McKendry RA.
Sci Rep. 2017 Sep 20;7(1):11971. doi: 10.1038/s41598-017-11887-6.

Unravelling the Molecular Basis of High Affinity Nanobodies against HIV p24: In Vitro Functional, Structural, and in Silico Insights.
Gray ER, Brookes JC, Caillat C, Turbé V, Webb BLJ, Granger LA, Miller BS, McCoy LE, El Khattabi M, Verrips CT, Weiss RA, Duffy DM, Weissenhorn W, McKendry RA.
ACS Infect Dis. 2017 Jul 14;3(7):479-491. doi: 10.1021/acsinfecdis.6b00189. Epub 2017 Jun 26.

A novel immuno-gold labeling protocol for nanobody-based detection of HER2 in breast cancer cells using immuno-electron microscopy.
Kijanka M, van Donselaar EG, Müller WH, Dorresteijn B, Popov-Čeleketić D, El Khattabi M, Verrips CT, van Bergen En Henegouwen PMP, Post JA.
J Struct Biol. 2017 Jul;199(1):1-11. doi: 10.1016/j.jsb.2017.05.008. Epub 2017 May 25.

Legomedicine-A Versatile Chemo-Enzymatic Approach for the Preparation of Targeted Dual-Labeled Llama Antibody-Nanoparticle Conjugates.
van Lith SA, van Duijnhoven SM, Navis AC, Leenders WP, Dolk E, Wennink JW, van Nostrum CF, van Hest JC.
Bioconjug Chem. 2017 Feb 15;28(2):539-548. doi: 10.1021/acs.bioconjchem.6b00638. Epub 2017 Jan 18.

Endogenous DKK1 and FRZB Regulate Chondrogenesis and Hypertrophy in Three-Dimensional Cultures of Human Chondrocytes and Human Mesenchymal Stem Cells.
Zhong L, Huang X, Rodrigues ED, Leijten JC, Verrips T, El Khattabi M, Karperien M, Post JN.
Stem Cells Dev. 2016 Dec 1;25(23):1808-1817. doi: 10.1089/scd.2016.0222. Epub 2016 Oct 27.

Dual anti-idiotypic purification of a novel, native-format biparatopic anti-MET antibody with improved in vitro and in vivo efficacy.
Godar M, Morello V, Sadi A, Hultberg A, De Jonge N, Basilico C, Hanssens V, Saunders M, Lambrecht BN, El Khattabi M, de Haard H, Michieli P, Blanchetot C.
Sci Rep. 2016 Aug 22;6:31621. doi: 10.1038/srep31621.

Hypoxia-Targeting Fluorescent Nanobodies for Optical Molecular Imaging of Pre-Invasive Breast Cancer.
van Brussel AS, Adams A, Oliveira S, Dorresteijn B, El Khattabi M, Vermeulen JF, van der Wall E, Mali WP, Derksen PW, van Diest PJ, van Bergen En Henegouwen PM.
Mol Imaging Biol. 2016 Aug;18(4):535-44. doi: 10.1007/s11307-015-0909-6.

Effective Inhibition of Bone Morphogenetic Protein Function by Highly Specific Llama-Derived Antibodies.
Calpe S, Wagner K, El Khattabi M, Rutten L, Zimberlin C, Dolk E, Verrips CT, Medema JP, Spits H, Krishnadath KK.
Mol Cancer Ther. 2015 Nov;14(11):2527-40. doi: 10.1158/1535-7163.MCT-14-0956. Epub 2015 Sep 8.

Camelid heavy chain only antibody fragment domain against β-site of amyloid precursor protein cleaving enzyme 1 inhibits β-secretase activity in vitro and in vivo.
Dorresteijn B, Rotman M, Faber D, Schravesande R, Suidgeest E, van der Weerd L, van der Maarel SM, Verrips CT, El Khattabi M.
FEBS J. 2015 Sep;282(18):3618-31. doi: 10.1111/febs.13367. Epub 2015 Jul 22.

Neutralization properties of simian immunodeficiency viruses infecting chimpanzees and gorillas.
Barbian HJ, Decker JM, Bibollet-Ruche F, Galimidi RP, West AP Jr, Learn GH, Parrish NF, Iyer SS, Li Y, Pace CS, Song R, Huang Y, Denny TN, Mouquet H, Martin L, Acharya P, Zhang B, Kwong PD, Mascola JR, Verrips CT, Strokappe NM, Rutten L, McCoy LE, Weiss RA, Brown CS, Jackson R, Silvestri G, Connors M, Burton DR, Shaw GM, Nussenzweig MC, Bjorkman PJ, Ho DD, Farzan M, Hahn BH.
mBio. 2015 Apr 21;6(2):e00296-15. doi: 10.1128/mBio.00296-15.

Molecular evolution of broadly neutralizing Llama antibodies to the CD4-binding site of HIV-1.
McCoy LE, Rutten L, Frampton D, Anderson I, Granger L, Bashford-Rogers R, Dekkers G, Strokappe NM, Seaman MS, Koh W, Grippo V, Kliche A, Verrips T, Kellam P, Fassati A, Weiss RA.
PLoS Pathog. 2014 Dec 18;10(12):e1004552. doi: 10.1371/journal.ppat.1004552. eCollection 2014 Dec.

Selection of VHHs under application conditions.
Dolk E, Verrips T, de Haard H.
Methods Mol Biol. 2012;911:199-209. doi: 10.1007/978-1-61779-968-6_13.