Single-domain antibody directed against Candidalysin
Target:
Candidalysin
Target description:
Candidalysin, also known as Ece1-III62-92K, is a 31-amino acid peptide toxin secreted by Candida albicans. In a large part of the human population, Candida albicans is a commensal fungus, but it can also causes serious mucosal and sometimes life-threatening systemic infections. Proteolytic cleavage of the larger protein Ece1 (extent of cell elongation 1, UniprotKB Q07730), creates the cytolytic pore-forming peptide toxin candidalysin, which is released upon hyphal morphogenesis of the pathogenic yeast, and it can intercalate in and damage host membranes of mostly epithelial cells. This damage triggers a calcium flux and a danger signaling pathway, which activates epithelial immunity.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with yeast and filamentous whole cells of Candida albicans and phage-display selection on immobilized candidalysin peptide.
Product details.
Specificity:
Candidalysin peptide from Candida albicans. Can neutralize candidalysin.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.2 kDa
Ext. Coeff. (ε):
31650 M-1cm-1
A280 at 1g/L:
2.1
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
