CD80, also called CTLA-4 counter-receptor B7, BB1 or B7 (UniProtKB P33681) is a costimulatory molecule essential for T-lymphocyte activation. It is a type I membrane protein from the immunoglobulin protein family with immunoglobulin-like extracellular domains consisting of a constant and variable subdomain. It is closely related to CD86 and both serve as a ligand for CD28 or CTLA-4. Whereas T-cell proliferation and cytokine production is induced by interaction with CD28, interaction of CD80 with CTLA-4 actually inhibits T-cell activation. CD80 is expressed on activated T-cells and activated B-cells but predominantly on antigen presenting cells like dendritic cells and macrophages. Surface expression of CD80 in macrophages is a marker for M1 polarization, a classically activated macrophage involved in promoting inflammation.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae. Immunization with cells. Phage-display selection on recombinant CD80 using total elution.
Product details.
Specificity:
Extracellular domain of human CD80.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.8 kDa
Ext. Coeff. (ε):
28545 M-1cm-1
A280 at 1g/L:
1.8
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
