Anti-Immunoglobulin Fc domain single-domain antibody.
Catalog no.:
Q96c
Clone name:
QFC-1F5
Product summary.
Product:
Single-domain antibody directed against Fc-domain
Target:
Fc-domain
Target description:
Immunoglobulin gamma (IgG) is the most abundant class of immunoglobulins in human plasma (10-20% of plasma proteins). The protein consists of two heavy chains and two light chains, forming a structure connected by the hinge region with two target binding arms (Fab domains when separate, or F(ab’)2 when connected via the hinge) and a constant region (Fc). The constant region contains sites for binding to immune receptors and proteins with amongst others the Fcγ-receptors (FcγR) (myeloid cells),2 C1q (complement system), and FcRn (IgG transport and half-life). IgG is present in four subclasses, in order of abundance the IgG1, IgG2, IgG3, and IgG4, with 90% similarity between each subclass. Each subclass has their own characteristics and function in the immune response. Immunoglobulins are widely used as molecular tools in biochemical and immunological research. Detection of Fc domains is required for assay development.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization and phage-display selection on immobilized recombinant human IgG Fc domain.
Product details.
Specificity:
Fc domain of human and cyno immunoglobulin 1, 2 and 4.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.3 kDa
Ext. Coeff. (ε):
27515 M-1cm-1
A280 at 1g/L:
1.8
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
