Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN or CD209, UniprotKB Q9NNX6), is a C-type lectin pathogen receptor that is expressed on the surface of macrophages and dendritic cells. It is involved in the primary immune response by taking up and degrading pathogens. On macrophages, DC-SIGN binds to pathogen associated molecular patterns (PAMPs), which triggers phagocytosis. On dendritic cells, it also recognized pathogens but in addition, interactions of DC-SIGN with intercellular adhesion molecules (ICAMs) also facilitate dendritic cell rolling along blood endothelium and CD4+ T cell activation. DC-SIGN is also used as target for vaccination.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with cancer cells. Phage-display selection on recombinant DC-SIGN using total elution.
Product details.
Specificity:
human DC-SIGN
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.3 kDa
Ext. Coeff. (ε):
23045 M-1cm-1
A280 at 1g/L:
1.5
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodiumazide is optional.
