Single-domain antibody directed against IR-Dye800CW
Target:
IR-Dye800CW
Target description:
IRDye 800CW is a water-soluble, near-infrared fluorescent dye that is often conjugated to biomolecules such as primary (single domain) antibodies, secondary antibodies, and peptides. These conjugates serve as probes in various applications, such as Western blotting, flow cytometry, immunohisto- or cytochemistry, In-Cell Western assays, protein arrays, in vivo imaging, and general optical probe development. IR-Dye800CW dye-conjugated probes are currently being investigated in several Phase I and Phase II clinical trials.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with labeled single-domain antibody and phage-display selection on conjugated protein using total elution.
Product details.
Specificity:
IR-Dye800CW
Formulation:
0.2 µm filtered solution in PBS. The products are equiped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation. This can for example be used to detect IRDye800CW-stained material with streptavidinHRP or to recolor it to other fluorescence wavelengths.
Mol. Weight:
14.6 kDa
Ext. Coeff. (ε):
27055 M-1cm-1
A280 at 1g/L:
1.9
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
