Human B7 homolog 3 (B7-H3) (UniProtKB Q5ZPR3, or CD276) is a member of the B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PD-L1 and PD-L2. B7 proteins are members of the immunoglobulin (Ig) superfamily, their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. As one of the exceptions, and as a result of exon duplication, B7-H3 is also expressed with 4 extracellular Ig-like domains. B7-H3 is normally expressed on antigen-presenting cells but is also a tumor antigen that is widely expressed among human solid tumors.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with cancer cells. Phage-display selection on recombinant protein using total elution.
Product details.
Specificity:
Extracellular domain of human B7-H3.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.9 kDa
Ext. Coeff. (ε):
28545M-1cm-1
A280 at 1g/L:
1.8
Storage:
Shipped on blue ice. Store at -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
