HIV-1 is an enveloped RNA lentivirus from the retroviridae family 1. The viral capsid contains RNA and enzymes and is formed by organized p24 protein (UniProtKB Q9WMv8). p24 is formed upon cleavage of the P55 Gag protein, which is encoded by the HIV-1 gag gene. Other cleavage products are p17, p7, and p6 proteins, which are required for virus assembly. p24 is an indicator of HIV-1 infection and viral load.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with recombinant proteins. Phage-display selection on captured recombinant protein using competitive or total elution.
Product details.
Specificity:
HIV Gag capsid protein p24 (CA) of 48/50 HIV strains with an apparent binding affinity of <1 nM.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
27 kDa
Ext. Coeff. (ε):
43680 M-1cm-1
A280 at 1g/L:
1.6
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
