Scavenger receptor cysteine-rich type 1 protein M130, also referred to as CD163 (UniProtKB Q86VB7). It is a single transmembrane protein with a short intracellular domain and 9 extracellular cysteine-rich scavenger receptor class B (SRCR) domains. CD163 is expressed on monocytes and macrophages and is the receptor for hemoglobin/haptoglobin complexes, facilitating clearance of these complex and hereby protecting from oxidative stress and potentially heme recycling. Surface expression of CD163 in macrophages is considered to be a marker for M2 polarization, a alternatively activated macrophage involved in tissue repair and immunosuppression. The extracellular domain of CD163 can be shedded and upon inflammatory stimuli and is thought to act anti-inflammatory.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae. Immunization with macrophages. Phage-display selection on recombinant CD163 using total elution.
Product details.
Specificity:
Extracellular domain of human CD163.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.9 kDa
Ext. Coeff. (ε):
35995 M-1cm-1
A280 at 1g/L:
2.3
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
