Epithelial cellular adhesion molecule (EpCAM, UniProtKB P16422), also known as TROP-1, is a 40 kDa type I transmembrane glycoprotein involved in Ca2+-independent cell adhesion, signaling, migration and proliferation. EpCAM was originally identified as a tumor-associated antigen because of its expression in a large number of cancers. EpCAM is expressed in basolateral cell membranes of epithelial cells. The protein consists of an 242 amino acid ectodomain that contains a signal peptide (SP) and two EGF-like subdomains, a 23 amino acid transmembrane domain and a cytoplasmic domain of only 26 amino acids. The 2nd EGF-like domain is also referred to as a thyroglobulin-like domain. This cytoplasmic domain of EpCAM interacts directly with α-actinin of the actin cytoskeleton.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with cancer cells. Phage-display selection on recombinant protein using total elution.
Product details.
Specificity:
Extracellular domain human EpCAM.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.3 kDa
Ext. Coeff. (ε):
24535 M-1cm-1
A280 at 1g/L:
1.6
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
