The Neural Cell Adhesion Molecule 1 (NCAM-1, UniProtKB P13591) is a
glycoprotein expressed on the membranes of neurons, glia and muscle cells.
However, it is also found to be expressed in cells of the immune system (NK cells, T-cells and dendritic cells). There are 4 types of NCAM-1 of which one variant is soluble, while the others or linked to the plasma mebrane via a GPI-anchor (120 kDa) or via a transmembrane domain (140 and 180 kDa). All types contain the 5x Ig-like domains and 2x Fn-like domains. NCAM-1 functions in cell-cell adhesion via binding to extracellular matrix protein agrin and several proteoglycans. In addition, its functioning is regulated via attachement of polysialylic acid to NCAM, generating PSA-NCAM.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified
from S. cerevisiae using affinity chromatography. Immunization with FSHD
patient material. Phage-display selection on cells and captured ectodomain
with total elution.
Product details.
Specificity:
Extracellular domain of human NCAM-1
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
14.8 kDa
Ext. Coeff. (ε):
31065 M-1cm-1
A280 at 1g/L:
2.1
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
