The transferrin receptor (TfR, CD71, UniProtKB P02786) is a type 2 single membrane spanning receptor (95kDa) that plays in imporant role in erythrocyte development and in the nerve system. TfR functions as a homodimer and regulates iron homeastasis via binding and cellular uptake of iron-loaded transferrin (Tf) via receptor-mediated endocytosis. The lower pH in endosomes releases the iron from the complex, allowing translocation to the cytoplasm. Upon recycling back to the plasma membrane, the apo-Tf dissociates from the rece ptor, which can then bind new iron-loaded Tf. In addition, TfR is able to trancytose proteins across endothelium/epithelium and the ectodomain can be secreted as circulating serum TfR.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with HUVEC cells. Phage-display selection on captured recombinant ectodomain with total elution.
Product details.
Specificity:
Extracellular domain of human TfR/CD71. Does not compete for Tf binding.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.5 kDa
Ext. Coeff. (ε):
27055 M-1cm-1
A280 at 1g/L:
1.7
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
