The epidermal growth factor receptor (EGFR/ErbB1/HER1, UniProtKB P00533) is one of the members of a family of 4 receptor tyrosine kinases (ErbB1 to 4). EGFR is a single membrane spanning protein of which binding of its natural ligands to the extracellular N-terminal domains I and III results in activation of the intracellular kinase domain (see figure). EGFR plays an important role in cell proliferation, survival and angiogenesis and it is overexpressed on and contributes to the development of a large number of cancers. EGFR is a validated tumor marker and an important therapeutic target.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with A431 cells. Phage-display selection on EGFR in immobilized membranes with competitive elution.
Product details.
Specificity:
Domain III of the extracellular domain of human EGFR. Q44 and Q86 bind to non-overlapping epitopes.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.2 kDa
Ext. Coeff. (ε):
37025 M-1cm-1
A280 at 1g/L:
2.4
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
