Bone Morphogenetic Proteins (BMPs) are TGF-β-like secreted signaling molecules that play important roles in tissue homeostasis and diseases, such as cancer. Although different, BMP2 (UniProtKB P12643) and BMP4 (UniProtKB P12644) originate from the same gene and show >80% sequence homology. Both BMP2 and BMP4 preferentially bind to the type I BMP receptors, BMPR1A (Alk3) and BMPR1B (Alk6), but can also signal through ActRI (Alk2).
Source:
Recombinant bivalent single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with recombinant BMP4. Phage-display selection on immobilized BMP4 with total elution.
Product details.
Specificity:
Binds to the ‘wrist hydrophobic groove’ on human BMP2 and BMP4a. Prevents binding of BMP4 to its receptor BMPR1a. Q35 and Q36 bind non-overlapping epitopes.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
29.3 kDa
Ext. Coeff. (ε):
41620 M-1cm-1
A280 at 1g/L:
1.4
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
