The Carbonic Anhydrase IX (CAIX), UniProtKB Q16790), isoform IX of the zinc enzyme carbonic anhydrase (α-CA family), is a single membrane spanning protein that functions as a dimer in pH regulation via the reversible hydration of carbon dioxide. CAIX has a relatively large extracellular domain (377 aa, consisting of a proteoglycan-like (PG) domain and catalytic domain) and small C-terminal intracellular (IC) domain (24 aa). Its expression is under the control of hypoxia-inducible factor 1α (HIF1α) 1,causes tumor acidification and is therefore used as one of the markers of hypoxia in tumors.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with HeLa cells grown under hypoxia. Phage-display selection on captured recombinant CAIX with total elution.
Product details.
Specificity:
Extracellular domain of human CAIX.
Formulation:
Lyophilized from a 0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
14.8 kDa
Ext. Coeff. (ε):
31525 M-1cm-1
A280 at 1g/L:
2.1
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
