The hepatocyte growth factor receptor (HGFR, cMet or Met, UniProtKB P08581) is a single membrane spanning receptor tyrosine kinase that is activated by hepatocyte growth factor (also known as scatter factor). Met is expressed at the cell surface as a 195 kDa hetero-dimeric protein. The extracellular part of Met contains three domain types: an N-terminal 7-bladed β-propellor-like SEMA domain (semaphorin), a PSI domain (plexin, semaphorin, integrin-like) and four IPT-domains (immuno-globulin-like). Blades 2-3 of the SEMA domain and IPT 3-4 interact with its natural ligand HGF.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with A431 cells. Phage-display selection on captured HGFR ectodomain with total elution.
Product details.
Specificity:
Blades 2-6 of the SEMA domain of human HGFR/Met. Competes for HGF binding.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.8 kDa
Ext. Coeff. (ε):
35535 M-1cm-1
A280 at 1g/L:
2.2
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
