HIV-1 is an enveloped RNA lentivirus from the retroviridae family 1. The surface of the virus expresses trimeric mushroom-shaped HIV-1-Env glycoprotein complexes that facilitate virus uptake via interaction with CD4 and CCR5 or CXCR4 on host cells. Env is a glycosylated trimer of non- linked gp120 and gp41 (UniprotKB Q53I19 formed upon proteolytic cleavage of the precursor gp160.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with recombinant proteins. Phage-display selection on captured recombinant protein using competitive or total elution.
Product details.
Specificity:
CD4 binding site on gp120.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.2 kDa
Ext. Coeff. (ε):
24535 M-1cm-1
A280 at 1g/L:
1.6
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
