The epidermal growth factor receptor 2 (ErbB2, HER2, Neu), UniProtKB P04626) is a single membrane spanning receptor tyrosine kinase that is activated by dimerization rather than ligand binding. HER2 is one of the 4 ErbB family members and is regarded as a proto-oncogene. It can heterodimerize with any of the other family members and dimerization results in activation and autophosphorylation of the C-terminal tyrosine residues. Overexpression of HER2 is observed in a large number of cancers and therefor serves as a target for tumor-imaging and therapy (e.g. cetuximab).
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with MCF7 cells. Phage-display selection on captured HER2 ectodomain with total elution.
Product details.
Specificity:
Extracellular domain of human ErbB2/Her.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15 kDa
Ext. Coeff. (ε):
28545 M-1cm-1
A280 at 1g/L:
1.9
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
