The CXC chemokine receptor type 4 (CXCR4 / fusin, UniProtKB P61073) is a 7
transmembrane spanning class A (rhodopsin-like) G protein-coupled receptor
(GPCR). Binding of the chemokine CXCL12/SDF1α activates heterotrimeric Gαi,
promoting cytoskeleton rearrangements and migration of e.g. immune cells to
sites of inflammation. CXCR4 is important during embryonic development and
regulates the homing and retention of hematopoietic stem cells in bone marrow.
Upregulation of CXCR4 and CXCL12 contributes to the progression and
metastasis of many tumor types. In addition, CXCR4 acts as a co-receptor for
entry of HIV-1 and HIV-2 into cells.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with CXCR4-containing
nanodiscs and cells. Phage-display selection on captured CXCR4-containing
lipoarticles with total elution.
Product details.
Specificity:
Extracellular part of human CXCR4 and competes for CXCL12 binding.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.9 kDa
Ext. Coeff. (ε):
24535 M-1cm-1
A280 at 1g/L:
1.5
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
