Single-domain antibody directed against ACKR3/CXCR7
Target:
ACKR3/CXCR7
Target description:
The atypical chemokine receptor 3 (ACKR3/CXCR7, UniProtKB P25106) is a 7-transmembrane spanning class A (rhodopsin-like) G protein-coupled receptor (GPCR). Binding of the chemokines CXCL12/SDF1α and CXCL11 activates biased signaling via β-arrestin. ACKR3 is expressed in several immune cells and is important for cell migration. Furthermore, it is expressed in the brain, kidneys, heart, and lungs, and plays a role during development. Besides this, ACKR3 is overexpressed in multiple cancer types, including breast-, lung-, and brain cancer, where it is involved in cancer progression and metastasis occurrence. ACKR3 is also involved in several autoimmune diseases and cardiovascular diseases.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with ACKR3-encoding plasmid DNA. Phage-display selection on captured ACKR3-containing lipoparticles with total elution.
Product details.
Specificity:
Extracellular part of human ACKR3 and competes for CXCL11 and CXCL12 binding.
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
14.6 kDa
Ext. Coeff. (ε):
25565 M-1cm-1
A280 at 1g/L:
1.75
Storage:
Shipped on blue ice. Store at -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
