The C-C chemokine receptor type 2 (CCR2 or CD192, UniProt P41597) is a 7-transmembrane spanning class A (rhodopsin-like) G protein-coupled receptor (GPCR). CCR2 has at least four human chemokine ligands, of which CCL2 is the most potent. CCR2 is predominantly expressed on monocytes and regulates (patho-)physiological inflammatory processes. Upregulated CCR2/CCL2 signaling is associated with cancer progression and metastasis, as well as inflammatory diseases.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with CCR2b-expressing lipoparticles. Phage-display selection on lipoparticles with total elution.
Product details.
Specificity:
Extracellular part of human CCR2b and competes with clinically tested conventional antibody Plozalizumab.
Formulation:
0.2 µm filtered solution in PBS. The product is equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15509 kDa
Ext. Coeff. (ε):
24535 M-1cm-1
A280 at 1g/L:
1.6
Storage:
Shipped on blue ice. Store at -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
Applications:
ELISA, flow cytometry, most likely antagonism (not tested)
