Single-domain antibody directed against complement protein C3
Target:
Complement protein C3
Target description:
The complement system plays a crucial role in immune defense. It is activated via three pathways: the classical pathway (CP), the lectin pathway (LP), and the alternative pathway (AP), resulting in opsonisation, chemoattraction of immune cells and target cell lysis. Complement component C3, a 185 kDa protein consisting of two disulfide linked chains, is a blood circulating protein with a central role in the complement system. During all three activation pathways C3 is cleaved by C3 convertases into C3a (9 kDa) and C3b (176 kDa). C3a acts as a chemoattractant and C3b is deposited onto pathogenic surfaces (opsonization), resulting in formation of subsequent convertase complexes and recognition by immune cells.
Source:
Recombinant bivalent single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with and phage-display selection on recombinant protein using total elution.
Product details.
Specificity:
Human C3, human C3b
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
15.2 kDa
Ext. Coeff. (ε):
31525 M-1cm-1
A280 at 1g/L:
2.1
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
