Single-domain antibody directed against human P-Selectin
Target:
Human P-Selectin
Target description:
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is a member of the Selectin family. It is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelet3 or Weibel-Palade bodies of endothelial cells, in response to a variety of inflammatory and thrombogenic stimuli. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1), and thereby mediates the adhesion of leukocytes to platelets and the endothelium. Additionally, the P-selectin/PSGL-1 interaction induces platelet activation thereby enhancing platelet aggregation and thrombus formation.
Source:
Immunization with and phage-display selection on purified recombinant human P-Selectin.
Recombinant monoclonal single-domain antibody (Lama glama), purified from HEK293-E 253 cells using Nickel excel Sepharose affinity chromatography.
Product details.
Specificity:
Human P-Selectin. Q117 inhibits the rolling of neutrophils and monocytes to adhered platelets and prevents the formation of platelet-neutrophil and platelet-monocyte complexes.
Formulation:
Myc-tagged single-domain antibody in PBS
Mol. Weight:
15.4 kDa
Ext. Coeff. (ε):
27566 M-1cm-1
A280 at 1g/L:
1.8
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
