Single-domain antibody directed against human glycoprotein 1b alpha
Target:
Human glycoprotein 1b alpha
Target description:
Glycoprotein 1b alpha (GPIbα), also known as CD42, is a transmembrane protein of 145 kDa. Together with GP1bβ, GPIX and GPV, it forms the non-covalent GP1b-V-IX complex on megakaryocytes and platelets. Platelet activation results in clearance of GPIb from the platelet surface followed by a slow reappearance to a normal surface expression level within an hour. The GP1b-V-IX complex functions as a receptor for von Willebrand factor, allowing platelet adhesion and platelet plug formation at sites of vascular injury. Additionally, GP1b contains a binding site for P-selectin, Mac-1, coagulation factor XI and XII, thrombin and high molecular-weight kininogen. Defects in GP1bα can cause serious bleeding diathesis, accompanied by morphological platelet anomalies. A gain-of-function mutation causes platelet-type von Willebrand disease.
Source:
Immunization with and phage-display selection on purified human human GP1bα.
Recombinant monoclonal single-domain antibody (Lama glama), purified from HEK293-E 253 cells using Nickel excel Sepharose affinity chromatography.
Product details.
Specificity:
Human GPIbα. Q114 binds the N-terminal leucine-rich repeats within GP1bα and interferes with the binding of vWF to GP1bα.
Formulation:
Myc-tagged single-domain antibody in PBS.
Mol. Weight:
14.8 kDa
Ext. Coeff. (ε):
19240 M-1cm-1
A280 at 1g/L:
1.3
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
