Single-domain antibody directed against tetraspanin CD63
Target:
CD63
Target description:
CD63 (melanoma-associated antigen, LAMP-3 or ME491) is a tetraspanin and a member of the transmembrane 4 superfamily. It is a cell surface-associated protein characterized by four transmembrane domains. As a tetraspanin, CD63 forms networks with a variety of proteins, called tetraspanin-enriched microdomains (TEMs). It is localized in different membranes of the cell, including the cell membrane and membranes of lysosomes and granules, including the platelet dense granules and the Weibel-Palade bodies of endothelial cells. The complex localization pattern suggests a tightly regulated trafficking and distribution process. CD63 is often used as a platelet activation marker, as cell surface expression increases due to platelet granule secretion after platelet activation.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from HEK293-E 253 cells using rmp-Protein A affinity chromatography and Ion Exchange Chromatography. Immunization with and phage-display selection on purified extracellular domain of recombinant human CD63 using total elution.
Product details.
Specificity:
Human CD63. Q112 targets a different sequence than clone Q111.
Formulation:
Tagless single-domain antibody in PBS.
Mol. Weight:
12.9 kDa
Ext. Coeff. (ε):
24897 M-1cm-1
A280 at 1g/L:
1.9
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
