Vimentin, UniProtKB P08670) is a class III intermediate filament (IF) protein, predominantly found in mesechymal cells. IFs are, besides actin and tubulin, one of the basic cytoskeletal component. Vimentin has a size of 466 amino acids or 57 kDa protein and is encoded by the VIM gene. Vimentin contains three linked coiled-coil domains and can be phosphorylated on serines and threonines. Vimentins can organize into unit-length filaments (ULFs) with a diameter of ~11 nm by longitudinal selfassociation of four octamers. Structures shown are derived from PDB files 3UF1 and 5WHF.
Source:
Recombinant monoclonal single-domain antibody (Lama glama), purified from S. cerevisiae using affinity chromatography. Immunization with muscle cells. Phage-display selection on captured Vimentin with total elution.
Product details.
Specificity:
Human vimentin
Formulation:
0.2 µm filtered solution in PBS. The products are equipped with a C-terminal C-Direct tag with an unpaired cysteine for directional conjugation.
Mol. Weight:
14.6 kDa
Ext. Coeff. (ε):
23045 M-1cm-1
A280 at 1g/L:
1.6
Storage:
Shipped on blue ice. Store at 4˚C or -20˚C (aliquots). Addition of 0.02% sodium azide is optional.
